Chemi-Verse™ LYN A Kinase Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Background: LYN A belongs to the Src family of intracellular membrane-associated tyrosine kinases (SFK) and is one of two splice variants. LYN proteins have a palmitoylation site, and are regulated by protein interactions and phosphorylation status. Phosphorylation by Csk (C-terminal Src kinases) results in the binding of LYN A SH2 domain to its SH3 domain, and inactivation. Dephosphorylation by proteins like CD45, or binding of the SH2/SH3 domain to ligands, releases the auto-inhibition and LYN A can undergo autophoshorylation and become active. It plays crucial roles in positive and negative-regulatory pathways in cells of the hematopoietic cell lineage, such as regulating integrin and FcRγ signaling in platelets. It can phosphorylate ITIMs (immunoreceptor tyrosine-based inhibitory motifs) and suppress ITAM (immunoreceptor tyrosine-based activation mofit) based signaling. Dysregulation of LYN proteins can result in antibody-mediated autoimmune diseases, and can potentiate leukemia, lymphoma and solid tumors. A deeper understanding of the role of LYN A versus LYN B in cell biology, and the impact of LYN A inhibition will be crucial for the development of effective cancer therapies where LYN A may play a role.

Contraindications: The final concentration of DMSO in the assay should not exceed 1%.

Description: The Chemi-Verse™ LYN A Kinase Assay Kit is designed to measure LYN A tyrosine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant LYN A kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Uniprot: P07948

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

References: Lingley E., 2012 Cell Communication and Signaling 10:21.
Brian B. and Freedman T., 2021 Endocrinology 162 (10: bqab152.

• Artikelnummer: BPS82224
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82224
• Verpackungseinheit: 96 reactions
• Mengeneinheit: PAK