DNA Polymerase γ Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Screen molecules that inhibit polymerization activity of DNA Polymerase γ in drug discovery and high throughput screening (HTS) applications.Determine compound IC₅₀.

Background: Polymerases are the enzymes responsible for synthesizing nucleic acids. Polymerase γ, also known as DNA polymerase subunit gamma, POLG or POLG1, belongs to the Family A of DNA polymerases and corresponds to the catalytic subunit of the mitochondrial DNA polymerase. It works in conjunction with POLG2 to replicate mitochondrial DNA. In addition to its DNA polymerase activity, it has a 3 ʹ-5 ʹ exonuclease and a 5 ʹdRP lyase activity for proofreading and repair. Mutations in polymerase γ cause mitochondrial dysfunction, as observed in SANDO (sensory ataxia neuropathy dysarthia and ophthalmoparesis), AHS (Alpers-Huttenlocher Syndrome) and MNGIE (mitochondrial neurogastrointestinal encephalopathy syndrome). Alternatively, the inhibition of DNA synthesis can be used as a therapeutic approach for diseases in which cell division or energetic needs are uncontrolled, such as cancer. Inhibitors such as gemcitabine have proved efficacious in the treatment of hematological and solid tumors (including breast cancer and ovarian cancer), particularly when combined with cisplatin. The use of cisplatin results in severe side effects, so the use of DNA inhibitors can also benefit patients ʹ care by allowing lower doses of platinum drugs to be used.  Further studies into DNA polymerase inhibitors will bring new therapy options to cancer patients.

Description: The DNA Polymerase γ Assay Kit is a fluorogenic assay designed to measure DNA Polymerase γ activity for screening and profiling applications, using AccuGreen™ as detection reagent. The kit comes in a convenient 96-well format and contains enough recombinant DNA Polymerase γ enzyme, DR Substrate 3, dNTP mix and buffer for 100 enzymatic reactions.                                                                                                                                 Note: AccuGreen™ High Sensitivity dsDNA Quantitation Kit is sold separately by Biotium.DNA Polymerase γ uses available nucleotides to synthesize a DNA strand complementary to the template strand, leading to the formation of double-stranded DNA molecule (dsDNA). The amount of dsDNA formed directly correlates to the polymerase activity and can quantified by the addition of the fluorescent DNA-binding dye which allows to distinguish dsDNA from ssDNA and free nucleotides. Since the dye emits fluorescence only when bound to the target dsDNA, the fluorometric readouts are low in the presence of a polymerase inhibitor.

Storage Stability: This assay kit contains DNA and will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Uniprot: P54098

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

References: Berdis A., 2017, Front. Mol. Biosci. 4:  doi.org/10.3389/fmolb.2017.00078

• Artikelnummer: BPS82098
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82098
• Green Labware: Nein
• Verpackungseinheit: 96 reactions
• Mengeneinheit: PAK