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Sialidase Fluorogenic Assay Kit
Sialidase Fluorogenic Assay Kit
Artikelnummer
BPS82154
Verpackungseinheit
96 reactions
Hersteller
BPS Bioscience
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Products from BPS Bioscience require a minimum order value above 400€
Applications: Enzyme kinetics studies and screening small molecule inhibitors for drug discovery and high-throughput screening (HTS) applications.
Background: Sialidases are glycosyl hydrolases that release α-2, 3-, α-2, 6- and α-2, 8-glycosidic linkages of terminal sialic acid residues from oligosaccharides, glycoproteins, and glycolipids. The Salmonella typhimurium sialidase NANH has been shown to have a high KM value (mM range) against polyvalent targets and plays a role in enhancement of NK cell-mediated antibody-dependent cellular cytotoxicity. Salmonella typhimurium sialidase is thought to be an immune-modulatory enzyme, therefore, its regulation may be important for therapy of diseases associated with disorders of the immune system.
Description: The Sialidase Fluorogenic Assay Kit is designed to measure Sialidase NANH (N-acylneuraminate glycohydrolase) activity for screening and profiling applications. It comes in a convenient 96-well format, with purified recombinant Salmonella typhimurium sialidase, Sialidase substrate, Sialidase assay buffer, and Stop Solution for 100 enzyme reactions. Sialidase inhibitor NCGC00063279 (N-Acetyl-2,3-dehydro-2-deoxyneuraminic acid) is also included as a control.The Sialidase fluorogenic substrate is incubated with Sialidase. Hydrolyzation of the Sialidase substrate releases a fluorophore (λexc/λem = 365 nm/445 nm). The increase in fluorescence at λ=445 nm is directly proportional to Sialidase activity.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Gray MA, et al., 2020, Nat. Chem. Biol. 16: 1376-1384Szijj PA, et al., 2023, Nat. Chem. doi: 10.1038/s41557-023-01280-4.
Applications: Enzyme kinetics studies and screening small molecule inhibitors for drug discovery and high-throughput screening (HTS) applications.
Background: Sialidases are glycosyl hydrolases that release α-2, 3-, α-2, 6- and α-2, 8-glycosidic linkages of terminal sialic acid residues from oligosaccharides, glycoproteins, and glycolipids. The Salmonella typhimurium sialidase NANH has been shown to have a high KM value (mM range) against polyvalent targets and plays a role in enhancement of NK cell-mediated antibody-dependent cellular cytotoxicity. Salmonella typhimurium sialidase is thought to be an immune-modulatory enzyme, therefore, its regulation may be important for therapy of diseases associated with disorders of the immune system.
Description: The Sialidase Fluorogenic Assay Kit is designed to measure Sialidase NANH (N-acylneuraminate glycohydrolase) activity for screening and profiling applications. It comes in a convenient 96-well format, with purified recombinant Salmonella typhimurium sialidase, Sialidase substrate, Sialidase assay buffer, and Stop Solution for 100 enzyme reactions. Sialidase inhibitor NCGC00063279 (N-Acetyl-2,3-dehydro-2-deoxyneuraminic acid) is also included as a control.The Sialidase fluorogenic substrate is incubated with Sialidase. Hydrolyzation of the Sialidase substrate releases a fluorophore (λexc/λem = 365 nm/445 nm). The increase in fluorescence at λ=445 nm is directly proportional to Sialidase activity.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Gray MA, et al., 2020, Nat. Chem. Biol. 16: 1376-1384Szijj PA, et al., 2023, Nat. Chem. doi: 10.1038/s41557-023-01280-4.
| Artikelnummer | BPS82154 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 82154 |
| Green Labware | Nein |
| Verpackungseinheit | 96 reactions |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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