GITRL, His-tag (Human) Recombinant

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Encompassing Amino Acids: 72-199

Applications: Useful for studying protein binding and for screening small molecules and antibodies.

Assay Conditions: GITR-biotin binding to GITRL was confirmed using the GITRL: GITR Biotinylated] Inhibitor Screening Assay Kit (BPS Cat. #72061).

Description: Human glucocorticoid-induced TNF-related ligand (GITRL), also known as AITRL, CD357L, or tumor necrosis factor ligand superfamily member 18 (TNFSF18). GenBank Accession No. NM_005092, a.a. 72-199 with an N-terminal His-tag, expressed in a HEK293 cell expression system. MW = 17.1 kDa. Note: this protein runs at a higher MW by SDSPAGE due to glycosylation.

Endotoxin Level: <1 EU/µg

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Genbank: NM_005092

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: Q9UNG2

Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.

Biosafety Level: Not applicable (BSL-1)

References: 1. Shevach, E.M. et al., Nat. Rev. Immunol. 2006; 6: 613-618.
2. Hanabuchi, S. et al., Blood. 2006; 107(9): 3617-3623.

• Artikelnummer: BPS71190
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 71190
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK