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Application: Study the impact of MSLN knockout.Use as a negative control for developing MSLN-directed CAR-T killing assays.
Background: Mesothelin (MSLN) is a glycophosphatidylinositol (GPI) linked cell-surface protein that is produced as a ~70 kDa precursor protein and cleaved by Furin protease to generate the ~40 kDa mature form. MSLN is frequently overexpressed in mesothelioma, ovarian, pancreatic, and non-small cell lung cancers, while its expression in normal tissues is restricted to the mesothelial lining. MSLN is a tumor-associated antigen and has been an attractive target for targeted immunotherapy approaches, including drug-conjugated antibodies and chimeric antigen receptor T cells (CAR-T Cells).The OVCAR3 cell line was isolated from a high-grade serous ovarian adenocarcinoma patient refractory to treatment with cisplatin. This cell line is highly tumorigenic and presents an abnormal karyotype. OVCAR3 cells are a widely model of ovarian cancer, particularly for drug resistance studies and hormonal therapy.
Description: MSLN Knockout OVCAR3 Cell Line is an ovarian cancer OVCAR3 cell line in which human MSLN (Mesothelin) has been genetically removed using CRISPR/Cas9 genome editing with a lentivirus encoding and stably expressing the CRISPR/Cas9 gene and sgRNA (single guide RNA) targeting human MSLN.
Host Cell Line: OVCAR3, ovarian adenocarcinoma cell line, adhering.
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains ˃1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Target: MSLN
Uniprot: Q13421
Unit Definition: Cell Line
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
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