English
PARP1 Homogenous Assay Kit
PARP1 Homogenous Assay Kit
Artikelnummer
BPS78438
Verpackungseinheit
384 rxns.
Hersteller
BPS Bioscience
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Products from BPS Bioscience require a minimum order value above 400€
Applications: Screen for molecules that inhibit PARP1 activity.
Contraindications: The PARP1 Homogenous Assay Kit is compatible with up to 1% final DMSO concentration.We recommend preparing the inhibitor in no higher than 5% DMSO solution in buffer and using 3 µl per well.Avoid green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.
Description: The PARP1 Homogeneous Assay Kit is designed to measure PARP1 activity for screening and profiling applications. PARP1 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP1 Homogeneous Assay Kit is a highly specific antibody that recognizes PARylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, a sample containing PARP1 enzyme is incubated with biotinylated substrate and NAD for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The PARP1 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone substrate, recombinant antibody (ADP-ribose binding reagent 1), PP-01 assay buffer, and purified PARP1 for 384 enzyme reactions.
Uniprot: P09874
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
Applications: Screen for molecules that inhibit PARP1 activity.
Contraindications: The PARP1 Homogenous Assay Kit is compatible with up to 1% final DMSO concentration.We recommend preparing the inhibitor in no higher than 5% DMSO solution in buffer and using 3 µl per well.Avoid green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.
Description: The PARP1 Homogeneous Assay Kit is designed to measure PARP1 activity for screening and profiling applications. PARP1 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP1 Homogeneous Assay Kit is a highly specific antibody that recognizes PARylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, a sample containing PARP1 enzyme is incubated with biotinylated substrate and NAD for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The PARP1 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone substrate, recombinant antibody (ADP-ribose binding reagent 1), PP-01 assay buffer, and purified PARP1 for 384 enzyme reactions.
Uniprot: P09874
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
| Artikelnummer | BPS78438 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 78438 |
| Green Labware | Nein |
| Verpackungseinheit | 384 rxns. |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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