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Encompassing Amino Acids: 110-476(end)
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done in Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 30 minutes. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Description: Human Ack1 (GenBank Accession No. NM_005781), a.a. 110-476(end) with N-terminal GST-tag, MW=68 kDa, expressed in Sf9 insect cells via abaculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Genbank: NM_005781
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q07912
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Eley, L. et al., Biochem. Biophys. Res. Commun. 371 (4), 877-882 (2008).
2. Grovdal, L.M. et al., Exp. Cell Res. 314 (6), 1292-1300 (2008).
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