Nucleofector

Nucleofector

The Principle of Nucleofector™ Technology:

By applying electrical pulses, small pores are generated short-term in the cell membrane. An interplay of preprogrammed Nucleofection protocols and coordinated reagents result in perfectly adapted conditions for a successful transfection for the particular cell type used. The substrate is not only taken up by the cytoplasm, but also by the nucleus due to the optimized conditions. This process allows high transfection rates with an efficiency of up to 99%.

We offer the following platforms:

  • For maximum flexibility: 4D Nucleofector (X, Y and LV units)
  • For medium-throughput: add-on 96-well shuttle
  • For high throughput: HT Nucleofector
  • The base unit: Nucleofector 2b

The Nucleofector kits contain special transfection cuvettes, Nucleofector reagents (buffer and supplement) and the control plasmid pmaxGFP. The buffer and its supplement are optimized specifically for respective primary cells.

Compatible Transfection Kits can be found here.

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  1. Electroporation cuvettes for bacteria, 1mm gap, 50 cuvettes
    Electroporation Cuvettes for Bacteria (1 mm gap)
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    Sku LONVKA-1001
    Manufact. SkuVKA-1001
    Packaging Unit50 cuvettes
  2. 96-well Nucleocuvette Plate (via MTA only)
    96-well Nucleocuvette Plate (via MTA only)
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    Sku LONVHNP-1001
    Manufact. SkuVHNP-1001
    Packaging Unit1 PC
  3. 96-well Shuttle Automation Package
    96-well Shuttle Automation Package
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    Sku LONSBA-1001
    Manufact. SkuSBA-1001
    Packaging Unit1 PC
  4. 4D-Nucleofector™ LogWare
    4D-Nucleofector LogWare
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    Sku LONSAAF-1001
    Manufact. SkuSAAF-1001
    Packaging Unit1 piece
  5. Inspection equipment 4D 16-well strip
    Inspection Equipment 4D-Nucleofector 16-well Strip
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    Sku LONAXP-1004
    Manufact. SkuAXP-1004
    Packaging Unit1 piece
  6. Inspection equipment 4D Single Cuvette
    Inspection Equipment 4D-Nucleofector Single Cuvette
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    Sku LONAXP-1003
    Manufact. SkuAXP-1003
    Packaging Unit1 piece
  7. Inspection Equipment 96-well Shuttle®
    Inspection Equipment 96-well Shuttle Device
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    Sku LONAXP-1002
    Manufact. SkuAXP-1002
    Packaging Unit1 piece
  8. Inspection Equipment Nucleofector® II
    Inspection Equipment Nucleofector II/2b Device
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    Sku LONAXP-1001
    Manufact. SkuAXP-1001
    Packaging Unit1 piece
  9. HT Nucleofector Installation
    384-well Nucleofector System Installation and Training
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    Sku LONAWT-1001
    Manufact. SkuAWT-1001
    Packaging Unit1 PC
  10. 4D-Nucleofector Commercial License
    4D-Nucleofector Commercial License
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    Sku LONAWF-1001
    Manufact. SkuAWF-1001
    Packaging Unit1 PC
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