Nucleofector

Nucleofector

The Principle of Nucleofector™ Technology:

By applying electrical pulses, small pores are generated short-term in the cell membrane. An interplay of preprogrammed Nucleofection protocols and coordinated reagents result in perfectly adapted conditions for a successful transfection for the particular cell type used. The substrate is not only taken up by the cytoplasm, but also by the nucleus due to the optimized conditions. This process allows high transfection rates with an efficiency of up to 99%.

We offer the following platforms:

  • For maximum flexibility: 4D Nucleofector (X, Y and LV units)
  • For medium-throughput: add-on 96-well shuttle
  • For high throughput: HT Nucleofector
  • The base unit: Nucleofector 2b

The Nucleofector kits contain special transfection cuvettes, Nucleofector reagents (buffer and supplement) and the control plasmid pmaxGFP. The buffer and its supplement are optimized specifically for respective primary cells.

Compatible Transfection Kits can be found here.

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  1. 1 mL Nucleocuvette™ Cartridge Box (2pcs)
    1 mL Nucleocuvette™ Cartridge Box (2pcs)
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    Sku LONV4LN-7002
    Manufact. SkuV4LN-7002
    Packaging Unit1 Piece
  2. LV Nucleocuvette™ Cartridge Box (weld.)
    LV Nucleocuvette™ Cartridge Box (weld.)
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    Sku LONV4LN-7020W
    Manufact. SkuV4LN-7020W
    Packaging Unit1 Piece
  3. LV Nucleocuvette™ Cartridge Box (1 pc)
    LV Nucleocuvette™ Cartridge Box (1 pc)
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    Sku LONV4LN-7020
    Manufact. SkuV4LN-7020
    Packaging Unit1 Piece
  4. 4D-Nucleofector™ LV Reservoir (weld.)
    4D-Nucleofector™ LV Reservoir (weld.)
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    Sku LONV4LR-1001W
    Manufact. SkuV4LR-1001W
    Packaging Unit1 Piece
  5. Electroporation cuvettes for bacteria, 1mm gap, 50 cuvettes
    Electroporation Cuvettes for Bacteria (1 mm gap)
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    Sku LONVKA-1001
    Manufact. SkuVKA-1001
    Packaging Unit50 cuvettes
  6. 96-well Nucleocuvette Plate (via MTA only)
    96-well Nucleocuvette Plate (via MTA only)
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    Sku LONVHNP-1001
    Manufact. SkuVHNP-1001
    Packaging Unit1 PC
  7. 96-well Shuttle Automation Package
    96-well Shuttle Automation Package - For use of the 96-well ShuttleTM Add-On in a liquid handling system
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    Sku LONSBA-1001
    Manufact. SkuSBA-1001
    Packaging Unit1 PC
  8. 4D-Nucleofector™ LogWare
    4D-Nucleofector LogWare
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    Sku LONSAAF-1001
    Manufact. SkuSAAF-1001
    Packaging Unit1 piece
  9. HT Nucleofector Installation
    384-well Nucleofector System Installation and Training
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    Sku LONAWT-1001
    Manufact. SkuAWT-1001
    Packaging Unit1 PC
  10. 4D-Nucleofector™ Y Unit Guarantee Extens ion (1 year)
    4D-Nucleofector Y Unit Guarantee Extension
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    Sku LONAWA-3001-Y
    Manufact. SkuAWA-3001-Y
    Packaging Unit1 PC
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