Immunohistochemistry

Immunohistochemistry

Immunohistochemistry (IHC) is an immune-based staining technique enabling antibody detection of specific antigens in tissue samples.

There are three main methods used in IHC: direct, indirect and enzyme „sandwich“ methods.

Direct IHC uses directly labelled (conjugated) primary antibodies while indirect IHC-detection uses unlabeled primary and labelled secondary antibodies. Both primary and secondary antibodies might be conjugated directly either to enzymes most commonly to horseradish peroxidase (HRP) or alkaline phosphatase (AP) or fluorophors. The sandwich method uses specific streptavidin or avidin binding reaction with biotin, which supports signal amplification, and might be preferable when detecting weakly-expressed antigens. Samples using enzymatic detection also need additionally enzyme substrates, which are enzymatically converted into stable chromogen products. These stable products can then be visualized under a light microscope.

We offer a broad range of primary and secondary antibodies, both unconjugated and conjugated to biotin, HRP, AP and various fluorophores. Available in our Webshop are also detection systems (avidin-biotin based, polymersystems), enzyme substrates, mounting media and buffers and solution for your successful immunohistochemical experiments.

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  1. SARS-CoV-2 (COVID-19) Spike FFPE 293T cell pellet slide
    SARS-CoV-2 (COVID-19) Spike FFPE 293T cell pellet slide
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    Sku GTX435744-5
    Manufact. SkuGTX435744-5
    Packaging Unit5 slides
  2. 293T FFPE Cell Pellet Block
    293T FFPE Cell Pellet Block
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    Sku GTX435670-1
    Manufact. SkuGTX435670-1
    Packaging Unit1 Block
  3. SARS-CoV-2 (COVID-19) Spike S2 FFPE Cell Pellet Block
    SARS-CoV-2 (COVID-19) Spike S2 FFPE Cell Pellet Block
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    Sku GTX435644-1
    Manufact. SkuGTX435644-1
    Packaging Unit1 block
  4. SARS-CoV-2 (COVID-19) Spike S1 FFPE Cell Pellet Block
    SARS-CoV-2 (COVID-19) Spike S1 FFPE Cell Pellet Block
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    Sku GTX435643-1
    Manufact. SkuGTX435643-1
    Packaging Unit1 block
  5. SARS-CoV-2 (COVID-19) Spike FFPE Cell Pellet Block
    SARS-CoV-2 (COVID-19) Spike FFPE Cell Pellet Block
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    Sku GTX435640-1
    Manufact. SkuGTX435640-1
    Packaging Unit1 block
  6. SARS-CoV-2 (COVID-19) Nucleocapsid FFPE Cell Pellet Block
    SARS-CoV-2 (COVID-19) Nucleocapsid FFPE Cell Pellet Block
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    Sku GTX435641-1
    Manufact. SkuGTX435641-1
    Packaging Unit1 block
  7. SARS-CoV-2 (COVID-19) Membrane FFPE Cell Pellet Block
    SARS-CoV-2 (COVID-19) Membrane FFPE Cell Pellet Block
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    Sku GTX435645-1
    Manufact. SkuGTX435645-1
    Packaging Unit1 block
  8. SARS-CoV-2 (COVID-19) Envelope FFPE Cell Pellet Block
    SARS-CoV-2 (COVID-19) Envelope FFPE Cell Pellet Block
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    Sku GTX435642-1
    Manufact. SkuGTX435642-1
    Packaging Unit1 block
  9. Vectashield® Plus Antifade Mounting Medium with DAPI
    Vectashield® Plus Antifade Mounting Medium with DAPI
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    Sku VECH-2000-10
    Manufact. SkuH-2000-10
    Packaging Unit10 ml
  10. Vectashield® Plus Antifade Mounting Medium with DAPI
    Vectashield® Plus Antifade Mounting Medium with DAPI
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    Sku VECH-2000-2
    Manufact. SkuH-2000-2
    Packaging Unit2 ml
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