Immunohistochemistry

Immunohistochemistry

Immunohistochemistry (IHC) is an immune-based staining technique enabling antibody detection of specific antigens in tissue samples.

There are three main methods used in IHC: direct, indirect and enzyme „sandwich“ methods.

Direct IHC uses directly labelled (conjugated) primary antibodies while indirect IHC-detection uses unlabeled primary and labelled secondary antibodies. Both primary and secondary antibodies might be conjugated directly either to enzymes most commonly to horseradish peroxidase (HRP) or alkaline phosphatase (AP) or fluorophors. The sandwich method uses specific streptavidin or avidin binding reaction with biotin, which supports signal amplification, and might be preferable when detecting weakly-expressed antigens. Samples using enzymatic detection also need additionally enzyme substrates, which are enzymatically converted into stable chromogen products. These stable products can then be visualized under a light microscope.

We offer a broad range of primary and secondary antibodies, both unconjugated and conjugated to biotin, HRP, AP and various fluorophores. Available in our Webshop are also detection systems (avidin-biotin based, polymersystems), enzyme substrates, mounting media and buffers and solution for your successful immunohistochemical experiments.

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  1. Rat 10% Washed Pooled Cells
    Rat 10% Washed Pooled Cells
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    Sku AVIOORA01071-100ML
    Manufact. SkuOORA01071-100ML
    Packaging Unit100 mL
  2. MOUSE SERUM (non-sterile)
    MOUSE SERUM (non-sterile)
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    Sku AVIOORA00669-50ML
    Manufact. SkuOORA00669-50ML
    Packaging Unit50 mL
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    PLASMA in Potassium EDTA
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    Sku AVIOORA00647-50ML
    Manufact. SkuOORA00647-50ML
    Packaging Unit50 mL
  4. Rat UTERUS
    Rat UTERUS
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    Sku AVIOORA00552-1EA
    Manufact. SkuOORA00552-1EA
    Packaging Unit1 Each
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    Rat TRACHEA
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    Sku AVIOORA00551-1EA
    Manufact. SkuOORA00551-1EA
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    Rat THYROID
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    Sku AVIOORA00550-1EA
    Manufact. SkuOORA00550-1EA
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    Rat THYMUS
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    Sku AVIOORA00549-1EA
    Manufact. SkuOORA00549-1EA
    Packaging Unit1 Each
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    Rat TESTICLE
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    Sku AVIOORA00548-1EA
    Manufact. SkuOORA00548-1EA
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    Rat SUBMAXILLARY
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    Sku AVIOORA00547-1EA
    Manufact. SkuOORA00547-1EA
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  10. Rat STOMACH
    Rat STOMACH
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    Sku AVIOORA00546-1EA
    Manufact. SkuOORA00546-1EA
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