Immunohistochemistry

Immunohistochemistry

Immunohistochemistry (IHC) is an immune-based staining technique enabling antibody detection of specific antigens in tissue samples.

There are three main methods used in IHC: direct, indirect and enzyme „sandwich“ methods.

Direct IHC uses directly labelled (conjugated) primary antibodies while indirect IHC-detection uses unlabeled primary and labelled secondary antibodies. Both primary and secondary antibodies might be conjugated directly either to enzymes most commonly to horseradish peroxidase (HRP) or alkaline phosphatase (AP) or fluorophors. The sandwich method uses specific streptavidin or avidin binding reaction with biotin, which supports signal amplification, and might be preferable when detecting weakly-expressed antigens. Samples using enzymatic detection also need additionally enzyme substrates, which are enzymatically converted into stable chromogen products. These stable products can then be visualized under a light microscope.

We offer a broad range of primary and secondary antibodies, both unconjugated and conjugated to biotin, HRP, AP and various fluorophores. Available in our Webshop are also detection systems (avidin-biotin based, polymersystems), enzyme substrates, mounting media and buffers and solution for your successful immunohistochemical experiments.

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  1. VectaPlex™ Antibody Removal Kit
    VectaPlex™ Antibody Removal Kit
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    Sku VECVRK-1000
    Manufact. SkuVRK-1000
    Packaging Unit1 Kit
  2. ABTS One Component HRP Microwell Substrate
    ABTS One Component HRP Microwell Substrate
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    Sku AVIGWB-B60A66-100
    Manufact. SkuGWB-B60A66-100
    Packaging Unit100ml
  3. ABTS One Component HRP Microwell Substrate
    ABTS One Component HRP Microwell Substrate
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    Sku AVIGWB-B60A66-1
    Manufact. SkuGWB-B60A66-1
    Packaging Unit1L
  4. DAB Immunohistochemistry Substrate
    DAB Immunohistochemistry Substrate
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    Sku AVIGWB-55335F-3
    Manufact. SkuGWB-55335F-3
    Packaging Unit3ml
  5. Tris/EDTA buffer, Heat-Induced Epitope Retrieval (10x, Tween20), Clear
    Tris/EDTA buffer, Heat-Induced Epitope Retrieval (10x, Tween20), Clear
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    Sku SANMON-APP920C
    Manufact. SkuMON-APP920C
    Packaging Unit100 ml
  6. Tris/EDTA buffer, Heat-Induced Epitope Retrieval (10x, Tween20), Blue color
    Tris/EDTA buffer, Heat-Induced Epitope Retrieval (10x, Tween20), Blue color
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    Sku SANMON-APP920B
    Manufact. SkuMON-APP920B
    Packaging Unit100 ml
  7. Citrate buffer, Heat-Induced Epitope Retrieval (10x, Tween20), Red color
    Citrate buffer, Heat-Induced Epitope Retrieval (10x, Tween20), Red color
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    Sku SANMON-APP919R
    Manufact. SkuMON-APP919R
    Packaging Unit100 ml
  8. Citrate buffer, Heat-Induced Epitope Retrieval (10x, Tween20), Clear
    Citrate buffer, Heat-Induced Epitope Retrieval (10x, Tween20), Clear
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    Sku SANMON-APP919C
    Manufact. SkuMON-APP919C
    Packaging Unit100 ml
  9. BrightDiluent, yellow antibody diluent
    BrightDiluent, yellow antibody diluent
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    Sku SANMON-APP917Y
    Manufact. SkuMON-APP917Y
    Packaging Unit125 ml
  10. BrightDiluent, red antibody diluent
    BrightDiluent, red antibody diluent
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    Sku SANMON-APP917R
    Manufact. SkuMON-APP917R
    Packaging Unit125 ml
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