Immunohistochemistry

Immunohistochemistry

Immunohistochemistry (IHC) is an immune-based staining technique enabling antibody detection of specific antigens in tissue samples.

There are three main methods used in IHC: direct, indirect and enzyme „sandwich“ methods.

Direct IHC uses directly labelled (conjugated) primary antibodies while indirect IHC-detection uses unlabeled primary and labelled secondary antibodies. Both primary and secondary antibodies might be conjugated directly either to enzymes most commonly to horseradish peroxidase (HRP) or alkaline phosphatase (AP) or fluorophors. The sandwich method uses specific streptavidin or avidin binding reaction with biotin, which supports signal amplification, and might be preferable when detecting weakly-expressed antigens. Samples using enzymatic detection also need additionally enzyme substrates, which are enzymatically converted into stable chromogen products. These stable products can then be visualized under a light microscope.

We offer a broad range of primary and secondary antibodies, both unconjugated and conjugated to biotin, HRP, AP and various fluorophores. Available in our Webshop are also detection systems (avidin-biotin based, polymersystems), enzyme substrates, mounting media and buffers and solution for your successful immunohistochemical experiments.

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  1. Streptavidin-HRP
    Streptavidin-HRP
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    Sku CEDCLSAHRP
    Manufact. SkuCLSAHRP
    Packaging Unit0,5 mg
  2. TMB Diluent (100 ml)
    TMB Diluent (100 ml)
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    Sku CEDCLAS15-DILUENT
    Manufact. SkuCLAS15-DILUENT
    Packaging Unit100 ml
  3. Human on Human Immunodetection Kit
    H.O.H.™ (Human on Human) Immunodetection Kit
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    Sku VECHOH-3000
    Manufact. SkuHOH-3000
    Packaging Unit1 Kit
  4. Twort's Gram Stain Kit
    Twort's Gram Stain Kit
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    Sku SCYTGS-1
    Manufact. SkuTGS-1
    Packaging Unit1 Kit
  5. Periodic Acid Schiff (PAS) with Light Green Stain Kit, CE-IVD
    Periodic Acid Schiff (PAS) with Light Green Stain Kit, CE-IVD
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    Sku SCYPASL-2
    Manufact. SkuPASL-2
    Packaging Unit1 Kit
  6. Hematoxylin, Weigert's Iron (Part B), CE-IVD
    Hematoxylin, Weigert's Iron (Part B), CE-IVD
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    Sku SCYHWI-B-125
    Manufact. SkuHWI-B-125
    Packaging Unit125 ml
  7. Hematoxylin, Weigert's Iron (Part A), CE-IVD
    Hematoxylin, Weigert's Iron (Part A), CE-IVD
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    Sku SCYHWI-A-125
    Manufact. SkuHWI-A-125
    Packaging Unit125 ml
  8. Peroxidase Substrate Kit AEC, 2 Components
    Peroxidase Substrate Kit AEC, 2 Components
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    Sku BILAE002
    Manufact. SkuAE002
    Packaging Unit1000 Tests
  9. SARS-CoV-2 (COVID-19) Spike FFPE 293T cell pellet slide
    SARS-CoV-2 (COVID-19) Spike FFPE 293T cell pellet slide
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    Sku GTX435744-5
    Manufact. SkuGTX435744-5
    Packaging Unit5 Slides
  10. 293T FFPE Cell Pellet Block
    293T FFPE Cell Pellet Block
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    Sku GTX435670-1
    Manufact. SkuGTX435670-1
    Packaging Unit1 block
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