Counterstain Reagents

Counterstains

Counterstains in IHC refer to staining solutions, which provide additional contract to the antibody. Some counterstains recognize specific cellular structures (e.g. nucleus), whereas others may stain whole cells. Selection of counterstains depends on their compatibility with the applied substrate and on the mounting medium.

Counterstains for chromogen detection

  • Hematoxylin – commonly used counterstain for intensive blue nuclear staining, suitable for most HRP and AP substrates. Works fine with aqueous as well as non-aqueous mounting media formulations.
  • Eosin Y – red counterstain staining cytoplasm
  • Methyl Green - light green counterstain – green nuclear staining solution, which might serve as an alternative to hematoxylin, to be used with non-aqueous mounting media.
  • Methylene blue – light blue counterstain staining cell nuclei, suitable for mounting with organic solvents-based media
  • Nuclear fast red - red aqueous counterstain – provides pink nuclear counterstain, suitable for non-aqueous mounting
  • Hematoxylin and eosin stain kit – nuclear and cytoplasmic counterstain based on Mayer´s hematoxylin
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  1. Lieb's Crystal Violet for Amyloid (0.5%)
    Lieb's Crystal Violet for Amyloid (0.5%)
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    Sku SCYLAM999
    Manufact. SkuLAM999
    Packaging Unit1000 ml
  2. Lieb's Crystal Violet for Amyloid (0.5%)
    Lieb's Crystal Violet for Amyloid (0.5%)
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    Sku SCYLAM500
    Manufact. SkuLAM500
    Packaging Unit500 ml
  3. Lieb's Crystal Violet for Amyloid (0.5%)
    Lieb's Crystal Violet for Amyloid (0.5%)
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    Sku SCYLAM250
    Manufact. SkuLAM250
    Packaging Unit250 ml
  4. Hematoxylin, Weigert's Iron (Part B)
    Hematoxylin, Weigert's Iron (Part B)
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    Sku SCYHWI-B-125
    Manufact. SkuHWI-B-125
    Packaging Unit125 ml
  5. Hematoxylin, Weigert's Iron (Part A)
    Hematoxylin, Weigert's Iron (Part A)
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    Sku SCYHWI-A-125
    Manufact. SkuHWI-A-125
    Packaging Unit125 ml
  6. Hematoxylin Qs
    Vector® Hematoxylin QS
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    Sku VECH-3404
    Manufact. SkuH-3404-100
    Packaging Unit100 ml
  7. Nuclear Fast Red
    Vector® Nuclear Fast Red
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    Sku VECH-3403
    Manufact. SkuH-3403-500
    Packaging Unit500 ml
  8. Methyl Green
    Vector® Methyl Green
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    Sku VECH-3402
    Manufact. SkuH-3402-500
    Packaging Unit500 ml
  9. Hematoxylin
    Vector® Hematoxylin
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    Sku VECH-3401
    Manufact. SkuH-3401-500
    Packaging Unit500 ml
  10. Wright-Giemsa Solution
    Wright-Giemsa Solution
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    Sku SCYWGS999
    Manufact. SkuWGS999
    Packaging Unit1000 ml
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