CloneID: S25-2
Antigen Long Description: This antibody was generated by immunizing a BALB/c mouse with KD03-GlcNAc-BSA.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody is specific for 2.4[2.8]2.4-linked Kdo tetrasaccharide. Which is a partial structure of chlamydial lipopolysaccharide (LPS).
Application Notes (Clone): An enzyme immunoassay was performed on LPS-BSA complexes and synthetic BSA-glycoconjugates using the mouse version of this antibody it showed reactivity to r595-207 LPS and to KD03-GlcNAc-BSA. Furthermore this antibody was used for immunofluorescence on L929 monolayers infected with C.trachomatis serotype L2 or C. psittaci 6BC at a dose resulting in >80% infected cells within 24 to 36 h, the antibody gave a positive result for both infections. Additionally western blots with LPS r595-207 and R595 as the antigen were performed using this antibody, it was postive for r595-207 and negative for R595 (Fu et al, 1992; pmid:1372290). An ELISA was performed on chlamydial LPS fragments using the mouse version of this antibody it bound most strongly to trisaccharides and tetrasaccharides (Evans et al, 2011; pmid:21543444). The binding affinity of the mouse version of this antibody was determined using SPR on α–Kdo-(2→8)-α–Kdo-(2→4)-α–Kdo, it bound with a Kd of 1,1μM (Müller-Loennies et al, 2000; pmid:10642603). SPR was performed on oligosaccharides using the mouse version of this antibody, for binding see Brooks et al, 2008. Furthermore ELISA was performed on neoglycoconjugates using this antibody, for reactivity see Brooks et al, 2008 (Brooks et al, 2008; pmid:18272175).
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