Anti-2.4(2.8)2.4-linked Kdo tetrasaccharide (S48-18)

Anti-2.4[2.8]2.4-linked Kdo tetrasaccharide [S48-18], Recombinant, IgG1 kappa, Mouse

CloneID: S48-18

Antigen Long Description: This antibody was generated by immunizing BALB/c mice with αKdo(2→4)αKdo(2→4)αKdo-BSA.

Buffer Composition: PBS with 0.02% Proclin 300.

Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7

Specificity Statement: This antibody is specific for 2.4[2.8]2.4-linked Kdo tetrasaccharide. Which is a partial structure of chlamydial lipopolysaccharide (LPS).

Application Notes (Clone): An ELISA was performed on neoglycoconjugates using the mouse version of this antibody, it showed the strongest binding to αKdo(2→4)αKdo(2→4)αKdo and αKdo(2→4)αKdo(2→4)αKdo(2→6)βGlcNAc. ELISA was also performed on LPS antigens and bacterial antigens using the mouse version of this antibody, it bound most strongly to Chl. psittaci EB and Chl. psittaci LPS (Brade et al, 2000; pmid:11521056). The scFv version of this antibody was used for an ELISA on neoglycoconugates, the antibody bound to 2.4/2.4PS4P and HS4P quite well but bound less well to 2.8/2.4PS4P (Gerstenbruch et al, 2010; pmid:20022906).

• SKU: ABAAb03581-1.1
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab03581-1.1
• Package Unit: 100 μg
• Quantity Unit: STK
• Reactivity: Various species
• Clonality: Recombinant
• Application: ELISA
• Isotype: IgG1 kappa
• Host: Mouse