Anti-2.4(2.8)2.4-linked Kdo tetrasaccharide (S73-2)

Anti-2.4[2.8]2.4-linked Kdo tetrasaccharide [S73-2], Recombinant, IgG1 kappa, Mouse

CloneID: S73-2

Antigen Long Description: This antibody was raised by immunizing mice with Kdo(2f8)[Kdo(2f4)]Kdo(2f4)Kdo(2f6)GlcNAc4P-(1f6)GlcNAcol-BSA and further immunizing them with Kdo(2f8)[Kdo(2f4)]Kdo-BSA.

Buffer Composition: PBS only.

Specificity Statement: This antibody is specific for 2.4[2.8]2.4-linked Kdo tetrasaccharide. Which is a partial structure of chlamydial lipopolysaccharide (LPS).

Application Notes (Clone): To screen hybridomas for this antibody ELISA was performed on neoglycoconjugates and immunofluorescence was performed on cells infected with either C. trachomatis or C. psittaci. Surface plasmon resonance was performed on Kdo(2f8)Kdo(2f4)KdoBSA (164, 1650, and 2460 RUs), Kdo(2f4)Kdo(2f4)Kdo-BSA, Kdo(2f4)Kdo BSA and BSA(Sigma), as a reference protein using the mouse version of this antibody (Brooks et al, 2010; pmid:20000757).

• SKU: ABAAb03582-1.1-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab03582-1.1-BT
• Package Unit: 1 mg
• Quantity Unit: STK
• Reactivity: Various species
• Clonality: Recombinant
• Application: Immunofluorescence, ELISA, Surface Plasmon Resonance (SPR)
• Isotype: IgG1 kappa
• Host: Mouse