CloneID: 8H9
Antigen Long Description: The antibody was originally generated by immunizing BALB/c mice with human neuroblastoma.
Buffer Composition: PBS only.
Uniprot Accession No.: Q5ZPR3
Specificity Statement: The antibody recognizes the 4Ig Domain Isoform of the Human B7-Homolog 3, 4Ig-B7H3. The epitope that the antibody recognizes appears to be restricted to tumors versus normal tissues.
Application Notes (Clone): The antibody was highly reactive with human brain tumors, childhood sarcomas, and neuroblastomas by immunohistochemistry. The antibody was nonreactive with normal human tissues. 4Ig-B7H3 was immunoprecipitated using this antibody (Modak et al., 2001; PMID: 11358824). In vitro characterization of radiolabeled 8H9 showed that (125)I-8H9 had a K(d) of 10.3nM with an estimated 115,000 binding sites on every HTB82 cell. Further, (125)I-8H9 was retained on the cell surface without significant internalization. In vivo targeting of 125I and 131Ilabeled 8H9 in human RMS xenografts was studied. 125I-8H9 could be used for tumor localization in animals. Instead, mice injected with 131I-8H9 showed a significant suppression in tumor volume (Modak et al., 2005; PMID: 16248769). Further, 131-Iodine-8H9 administered through the Ommaya had favorable pharmacokinetics in non-human primates with minimal toxicities. 31-Iodine-8H9 was used in a phase I study with brain tumors, the antibody was safe and might have clinical utility when added to salvage therapy using conventional modalities in the treatment of 8H9-positive LM/CNS cancers. The antibody detected 4Ig-B7H3 under native conditions in LAN-1, HTB82 and U2OS but not in Daudi cells by western blot analysis. The antibody was unable to recognise the antigen under reducing conditions in Western blot analysis (US20100143245A1).
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