CloneID: GT1-mAb2
Antigen Long Description: This antibody was obtained by sequencing a polyclonal goat IgG raised against a peptide with the sequence C-TGPPAKKAISELP.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P55008
Specificity Statement: This antibody is specific to
EBP05419, an immunizing peptide with the sequence C-TGPPAKKAISELP. This peptide was used in the production of the
polyclonal Goat Anti-AIF1 antibody from which this antibody is derived.
Application Notes (Clone): This antibody (GT1-mAb2) was one of two monoclonal antibodies discovered by analyzing a polyclonal antibody (GT-1) sample. Briefly, de novo sequencing of GT-1 resulted in the full-length sequences of two distinct monoclonal antibodies (GT1-mAb1 and GT1-mAb2) in both framework and CDR regions. Both antibodies were recombinantly expressed by Absolute Antibody in HEK293 cells and tested in various assays. GT1-mAb2 was tested against the peptide antigen in an ELISA, and it showed activity comparable to that of the original polyclonal antibody. GT1-mAb2 was found to bind the target peptide in a non-competitive manner with GT1-mAb1 while being efficiently blocked by the original polyclonal antibody, proving that both monoclonal antibodies bind distinct epitopes and that GT1-mAb2 possess binding characteristics of the input polyclonal antibody. Furthermore, GT1-mAb2 was found to be compatible with immunofluorescence (IF) and flow cytometry (FC), although with an FC signal observedly less distinct than that of GT1-mAb1.
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