CloneID: B4m3
Antigen Long Description: This antibody was generated by first panning the Nkuku® library [26] against 20 μg/ml of the M. tuberculosis 16 kDa antigen. Second a sublibrary was created using error-prone PCR. Finally the mutant library was panned against 16 kDa M. tuberculosis antigen.
Buffer Composition: PBS only.
Chimeric Use Statement: This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Chicken scFv format, for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P9WMK1
Specificity Statement: This antibody is specific for the HspX antigen from Mycobacterium tuberculosis. This antigen acts as a chaperone, as it has a significant ability to suppress the thermal denaturation of alcohol dehydrogenase. Mycobacterium tuberculosis causes the disease tuberculosis.
Application Notes (Clone): The binding of the scFv version of this antibody to the antigen was characterised by ELISA. Furthermore, surface plasmon resonance was used to determine the binding kinetics of this antibody on 16 kDa protein of M. tuberculosis (Sixholo et al, 2011; pmid:21349739).
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