CloneID: EnL5
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing female white leghorn (Gallus domesticus) chickens with 100 μg purified α-enolase in an equal volume of Freund's complete adjuvant by an intramuscular injection. The total RNA was extracted from the enlarged spleens for scFv antibody library construction. This clone was isolated by panning the scFv library against alpha-enolase.
Buffer Composition: PBS only.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG format, created for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P06733
Specificity Statement: This antibody binds alpha-enolase, which is a glycolytic enzyme expressed in most tissues. It catalysis the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, it is involved in various processes such as growth control, hypoxia tolerance and allergic responses
Application Notes (Clone): The binding specificity of this antibody for alpha-enolase was done using ELISA. This antibody was also used for detection of α-enolase expressed in the PE089 cells using flow cytometry. This antibody was also used for immunofluorescent and immunocytochemical staining of α-enolase molecules expressed in PE089 cells (PMID: 20655599).
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