CloneID: A5A3
Antigen Long Description: The original antibody was generated by immunizing a barnevelder chicken with an antibody antigen cocktail comprising recombinant purified human antigens annexin XI, CENP-B, Topo, Jo-1, Ro60, Rpp38 and U1A in complete Freund's adjuvant. B cells that were harvested from the bone marrow and peripheral blood lymphocytes of immunized chickens was used to generate recombinant scFv phage display libraries. The specific clone was identified by panning the library against specific antigen.
Buffer Composition: PBS only.
Chimeric Use Statement: This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Chicken scFv format for improved compatibility with existing reagents assays and techniques.
Uniprot Accession No.: P50995
Specificity Statement: The antibody is specific for human annexin XI. Annexin XI binds specifically to calcyclin in a calcium-dependent manner and it is required for midbody formation and completion of the terminal phase of cytokinesis.
Application Notes (Clone): The binding characterization of this antibody was done using ELISA. This antibody is also capable of recognizing human annexin XI in a western blot assay (Hof et al., 2008; PMID: 18062792).
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