Anti-Beta Lactamase (cAbTEM13)

Anti-Beta Lactamase [cAbTEM13], Recombinant, IgG-Fc Fusion, Rabbit

CloneID: cAbTEM13

Antigen Long Description: The original antibody was generated by immunizing adult dromedary (Camelus dromedarius) with TEM-1 β-lactamase.

Origin Pub PMID: 11557473

Buffer Composition: PBS only.

Uniprot Accession No.: P62593

Specificity Statement: This antibody binds an enzyme called beta-lactamase produced by bacteria, which catalysis the breaking of the beta-lactam ring of beta-lactam antibiotics such as penicillin, cephalosporin, cephamycin and monobactam through hydrolysis. The breaking of the beta-lactam ring deactivates the antibacterial properties of the antibiotic molecule and provides antibiotic resistance in bacteria.

Application Notes (Clone): The binding reactivity of this antibody to TEM-1 beta-lactamase was determined using ELISA. This antibody was capable of inhibiting beta-lactamase activity in vitro with an IC50 of 0.08 μM. The in vivo inhibition activity of this antibody to inhibit surface-exposed TEM-1 and prevent bacterial growth was assessed by preincubating cells expressing TEM-1 with increasing amounts of the antibody fragment. It was reported that when cells were preincubated with cAbTem13 before plating lost their ability to neutralize the antibiotic ampicillin (PMID: 11557473).

• SKU: ABAAb03503-23.159-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab03503-23.159-BT
• Package Unit: 1 mg
• Quantity Unit: STK
• Reactivity: Escherichia Coli
• Clonality: Recombinant
• Application: ELISA
• Isotype: IgG-Fc Fusion
• Host: Rabbit