CloneID: scFv 18
Antigen Long Description: This antibody was generated by immunizing a BALB/c mouse with 0.1 ml of the commercial pertussis vaccine Quandracel. From this DNA a phage display library was created and panned against B. pertussis whole cells so find this clone.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This full-length, reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P14283; P35077
Specificity Statement: This antibody is specific for pertactin and filamentous hemagglutinin of Bordetella pertussis. Filamentous hemagglutinin is a member of a two partner secretion pathway (TPS) in which it mediates the secretion of filamentous hemagglutinin FHA (fhaB). Pertactin may have a role in bacterial adhesion, and thus play a role in virulence.
Application Notes (Clone): The scFv version of this antibody was used for an ELISA on filamentous hemagglutinin (FHA), pertactin (PRN) and B. pertussis cells. The antibody was positive very every antigen tested. The scFv version of this antibody was tested for its ability to inhibit B. pertussis binding to HEp-2 cells, it did not show any inhibition. (Hussein et al, 2007; pmid:17724067).
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