Anti-Bordetella pertussis cells (scFv 18)

Anti-Bordetella pertussis cells [scFv 18], Recombinant, scFv fragment (His)

CloneID: scFv 18

Heavy Chain modification: ScFv

Antigen Long Description: This antibody was generated by immunizing a BALB/c mouse with 0.1 ml of the commercial pertussis vaccine Quandracel. From this DNA a phage display library was created and panned against B. pertussis whole cells so find this clone.

Buffer Composition: PBS only.

Uniprot Accession No.: P14283; P35077

Specificity Statement: This antibody is specific for pertactin and filamentous hemagglutinin of Bordetella pertussis. Filamentous hemagglutinin is a member of a two partner secretion pathway (TPS) in which it mediates the secretion of filamentous hemagglutinin FHA (fhaB). Pertactin may have a role in bacterial adhesion, and thus play a role in virulence.

Application Notes (Clone): The scFv version of this antibody was used for an ELISA on filamentous hemagglutinin (FHA), pertactin (PRN) and B. pertussis cells. The antibody was positive very every antigen tested. The scFv version of this antibody was tested for its ability to inhibit B. pertussis binding to HEp-2 cells, it did not show any inhibition. (Hussein et al, 2007; pmid:17724067).

• SKU: ABAAb03579-30.11-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab03579-30.11-BT
• Package Unit: 500 μg
• Quantity Unit: STK
• Reactivity: Various species
• Clonality: Recombinant
• Application: ELISA, Functional Assay
• Isotype: scFv fragment (His)