CloneID: 3A5
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was raised by immunizing a BALB/c mouse with a recombinant capsid protein of PCV2 expressed by a baculovirus vector
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted mouse IgG2a Fc Silent™ antibody, based on the original mouse IgG2a format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: O56129; F5A4Z4
Specificity Statement: This antibody is specific for the epitopes found at the topmost edges around the 5-fold axes of the PCV2, Cap with each epitope covering 18 amino acids from two adjacent Cap proteins.
Application Notes (Clone): The original antibody was used for an ELISA on PCV2-positive serum (Huang et al., 2011; PMID:20923690), on PCV2a/LG-infected PK15 cells cultures (Huang et al., 2019; PMID:30863876), and on purified PCV2 (Bian et al., 2021; PMID:34959519). Further this antibody was used for immunohistochemistry on inguinal lymph nodes of PCV2-inoculated pigs (Huang et al., 2016; PMID:27020283) (Huang et al., 2019; PMID:30863876). This antibody was used for an neutralization assay on PCV2a/LG. PCV2a/LG was incubated with this antibody after which the mixture was added to PK15 cells (Huang et al., 2020; PMID:32075932) (Yu et al., 2022; PMID:35945364). This antibody was also used for immunofluorescence to test the absorption of PCV2 to PK15 cells. The structure of the original antibody was determined using cryo-EM (Huang et al., 2020; PMID:32075932). This antibody was used for purification of proteins from PCV2a/LG (Bian et al., 2021; PMID:34959519)
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