CloneID: Nb15
Antigen Long Description: The original antibody was generated by immunizing Bactrian camel (Camelus bactrianus) with recombinant PCV2-Cap protein, followed by antibody library construction and phage display-based selection.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original VHH format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: O56129
Specificity Statement: The antibody binds to PCV2 Cap protein. There was no cross-reaction when detecting positive sera against other porcine viruses. the M2 cytoplasmic domain interacts directly with mammalian host cell proteins (e.g., in the autophagy pathway) and plays an important role in regulating viral replication.
Application Notes (Clone): The ability of this antibody (VHH) to recognize PCV2 Cap protein was confirmed by indirect ELISA analysis. The antibody was used to develop a competitive ELISA for detecting PCV2 antibody levels in porcine serum samples (Mu et al, 2021; pmid:33526021).
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