Anti-Capsid protein VP1 (Nano-25)

Anti-Capsid protein VP1 [Nano-25], Recombinant, VHH, Camelid

CloneID: Nano-25

Heavy Chain modification: His-tagged

Antigen Long Description: The original antibody was generated by immunizing a single alpaca with the GII.10 virus-like particle protein.

Origin Pub PMID: 25520510

Buffer Composition: PBS only.

Uniprot Accession No.: Q5F4T5

Specificity Statement: This antibody is specific for the GII.10 group of the protruding (P) domain of the Norwalk virus capsid protein VP1. The P1 subdomain binding site is occluded on intact particles, yet this antibody binds intact norovirus VLPs and virions.

Application Notes (Clone): The reactivity of the original antibody (alpaca VHH) against norovirus VLPs and P domains was determined using ELISA. Using isothermal titration calorimetry, a K_d of 28 nM was measured between the antibody and the GII.10 P domain. The crystal structure of this antibody in complex with norovirus GII.10 P domain was determined (Koromyslova & Hansman, 2015; PMID: 25520510). The epitope of this antibody was further characterized in X-ray crystallography and cryo-EM (Koromyslova & Hansman, 2017; PMID: 29095961).

• SKU: ABAAb04579-34.11-BS
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab04579-34.11-BS
• Package Unit: 1 mg
• Quantity Unit: STK
• Reactivity: Virus
• Clonality: Recombinant
• Application: ELISA, Crystallography, Electron microscopy (EM)
• Isotype: VHH
• Host: Camelid