CloneID: Nec8-4116-8
Antigen Long Description: The original antibody was generated be immunizing KM mice with an emulsion prepared by mixing the Nectin-2ED-Fc protein and Nectin-2ED-FLAG protein.
Buffer Composition: PBS only.
Uniprot Accession No.: Q92692
Specificity Statement: This antibody binds human CD112 (Nectin-2) and cross reacts with cynomolgus monkey CD112. It is also capable of binding AN77-78PD, G113R and H128R mutants of human CD112. Nectin-2 acts as a modulator of T-cell signaling. It can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation.
Application Notes (Clone): The binding of this antibody to CD112 or Nectin-2 expressed on the surface of CHO cells was determined using whole cell ELISA. The cross reactivity of this antibody for cynomolgus monkey CD112 expressed on CHO-K1 cells was checked using flow cytometry. This antibody shows good ADCC activity against OV-90, MDA-MB-231, NCI-H1299, Du145 and U937 cell lines. The in-vivo activity of this antibody was checked in SCID mouse models for treating lung metastasis with MDA-MB-231 human breast cancer cell line and nude mice models for treating liver metastasis with OV-90 human ovarian cancer cell line (US20100008928).
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