CloneID: OX86
Heavy Chain modification: Fc Silent™
Antigen Long Description: This antibody was raised against the recombinant mouse OX40 protein.
Buffer Composition: PBS only.
Chimeric Use Statement: This is a chimeric antibody created to reduce immunogenicity during in vivo applications.
Uniprot Accession No.: P47741
Specificity Statement: This antibody recognises murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes. It is a member of TNFR-superfamily of receptors and acts as a costimulatory molecule involved in long-term T-cell immunity.
Application Notes (Clone): OX86 antibody, in its original rat IgG1 format, acts as an OX40 agonist in an Fc-dependent manner and has been widely used in studies investigating the role of OX40 in tumor inhibition (Hirschhorn-Cymerman et al., 2009). Studies comparing two recombinant murine Fc versions (IgG1 and IgG2a) elucidated the importance of the FcR engagement in the antitumor activity of this antibody; the study demonstrated that a stronger murine Fc (in an IgG2a version) was more effective in inducing a CT26 syngeneic tumor regression as well as it led to a stronger Treg depletion in the tumor in comparison with a murine IgG1 version (Metzger et al., 2016). In another study two versions of the OX86 antibody (with a fully functional Fc fragment and with an impaired Fc) and showed that Fc-dependent activity was necessary in inducing systemic antitumor activity (Sagiv-Barfi et al., 2018). FC: This antibody was also used in flow cytometry to stain mouse CD4 and CD8 single-positive cells (al-Shamkhani et al., 1996). FC: Blanquiceth et al. (2016) used this antibody in flow cytometry as well to analyse cells coming from mediastinal lymph nodes and lung tissue. IHC: OX86 antibody was utilised in immunohistochemistry staining of gut tissue (Peyer's patches and lamina propria samples) in order to identify OX40-positive cells in colitic mice (Higgins et al., 1999).
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