CloneID: GK1.5
Antigen Long Description: The antibody was raised by immunizing a Lewis rat with an i.v. injection of 30 x 10' Ficoll-Hypaque-purified cells from the cloned CTL line 243/2.5
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P06332
Specificity Statement: This antibody is specific for CD4. CD4 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.
Application Notes (Clone): The effect of this antibody on the PFC response was measured by treating spleen cells from PC-FGG-primed BALB/c mice with this antibody (Dialynas et al, 1983; pmid:6415170). Immunofluorescence was preformed preformed on spleen cells from PC-FGG-primed BALB/c mice with this antibody (Dialynas et al, 1983; pmid:6415170). An flow cytometry experiment was preformed on spleen cells from PC-FGG-primed BALB/c mice with this antibody (Dialynas et al, 1983; pmid:6415170). The effect of this antibody on antigen-specific release of IL2 by mouse T cell hydridomas (Wilde et al, 1983; pmid:6195255). ELISA was preformed with this antibody on calf fetal serum (Wilde et al, 1983; pmid:6195255). The effect of the antibody on antigen-specific binding by T cell hybridoma AODH-3.4 was determined (Wilde et al, 1983; pmid:6195255). ELISA was preformed on murine CD4 using this antibody (Freise et al, 2017; pmid:27966069). The biodistribution of this antibody in mice was determined (Freise et al, 2017; pmid:27966069). Flow cytometry was preformed on blood, Thymus, lymph nodes, and spleen cells from mice with this antibody (Freise et al, 2017; pmid:27966069). Mouse C57BL/6 splenocytes were used in a proliferation assay with this antibody (Freise et al, 2017; pmid:27966069). Mouse Blood, spleen, and lymph node lymphocyte were used for flow cytometry using this antibody (Rice et al, 1995; pmid:7539027). Multiple organs from mice were used for immunohistochemistry using this antibody (Rice et al, 1995; pmid:7539027). Flow cytomic detection was used to detect T cell depletion using this antibody (Rashid et al, 1992; pmid:1538121).
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