CloneID: 101-1D2
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was raised by immunizing mice with a mixed leukocyte culture.
Buffer Composition: PBS only.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P32942
Specificity Statement: This antibody recognizes CD50 domain 1.
Application Notes (Clone): The original version of this antibody was used for immunofluorescence on T cells, B cells and non lymphoic cell lines (Juan et al., 1993; PMID:8325327), on thymocytes (Martinez-Caceres et al., 1996; PMID:9008304), and on peripheral blood stimulated or unstimulated cells . Furthermore this antibody was used for flow cytometry on thymocytes (Martinez-Caceres et al., 1996; PMID:9008304), on peripheral blood stimulated or unstimulated cells (Vilella et al., 1990; PMID:1710077), and on T cells (Vilardell et al., 1998; PMID: 9672149). This antibody was also used for immunoprecipitation on PMBCs to determine whether CD50 and ICAM-3 are the same protein (Juan et al., 1993; PMID:8325327), and on peripheral blood stimulated or unstimulated cells to prove that 101–1D2 and 140–11 recognize the same molecule (Vilella et al., 1990; PMID:1710077). This antibody was used for immunohistochemistry on human neoplasms to study the expression of CD50 (Terol et al., 1996; PMID: 8946680). The ability of this antibody to inhibit cell-mediated lympholysis was tested in an inhibition assay on PMBCs was tested, it showed it was able to inhibit lympholysis (Vilella et al., 1990; PMID:1710077). Finally this antibody was used for western blot on immunoprecipitated proteins. This experiment shows that the CD50 protein coprecipitates with a 56-kD lck protein and a 59-kD fyn PTK (Juan et al., 1994; PMID:7515097).
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