CloneID: 3DF1
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice intraperitoneally with Citrus tristeza virus (isolate T-308) preparation.
Origin Pub PMID: 18943374
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q00686
Specificity Statement: This antibody binds the p25 major coat protein of the Citrus tristeza virus (CTV). This antibody cannot bind the CTV isolate T-397-P. Tristeza is the most important virus disease of citrus worldwide. It is caused by citrus tristeza virus (CTV), a closterovirus that is naturally spread in the fields by aphids in a semipersistent manner and is also easily transmitted by grafting. CTV is a monopartite, single stranded RNA virus encapsulated in a coat protein.
Application Notes (Clone): This antibody can be used for the detection of Citrus tristeza virus in crude extracts of plants infected with isolates T-300 or T-308 using ELISA and double antibody sandwich ELISA. This antibody reacted with 20 CTV isolates from different Spanish citrus areas as well as with CTV isolates from California (T-514) and two unclassified from Iran and Vietnam in an ELISA (Vela et al., 1986). Transgenic plants expressing this antibody showed both a delay in symptom appearance and attenuation of symptom intensity. Overall, 40–60% of the plants were resistant, as revealed by the absence of virus antigens in tissue-print ELISA (PMID: 20204695).
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