Anti-Clusterin (16B5)

Anti-Clusterin [16B5], Recombinant, IgG1 kappa, Human

CloneID: 16B5

Heavy Chain modification: Fc Silent™

Buffer Composition: PBS only.

Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Uniprot Accession No.: P10909

Specificity Statement: The antibody binds clusterin on the EMT-epitope.

Application Notes (Clone): The effect of the original format of the antibody was evaluated on lung metastasis in BALB/c mice with orthotopically implanted 4T1 cells. The antibody was able to significantly reduce lung metastasis (Lenferink et al., 2010; PMID: 19935703). A humanized version of the antibody was constructed. The humanized version of the antibody inhibited the EMT-inducing activity of clusterin on A549 NSCLC cells. Immunofluorescence was performed on A549 cells using this antibody to monitor E-cadherin expression. The combination of the humanized version of the antibody with erlotinib resulted in an enhanced anti-tumour effect (H1299 and H460 cells) (US9822170). AB-16B5 is a humanized IgG2 version of the antibody which specifically binds to tumour-associated clusterin and inhibits its activity. This antibody showed a good tumour inhibitory effect in in vivo application (Tremblay et al.; 2010). A phase I and phase II trial to assess safety and tolerability of AB-16B5 for the treatment of solid tumours was performed (Ferrario et al., 2017; NCT04364620).

• SKU: ABAAb03504-10.3-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab03504-10.3-BT
• Package Unit: 1 mg
• Quantity Unit: STK
• Reactivity: Human
• Clonality: Recombinant
• Application: Immunofluorescence, In Vivo Assay, Inhibition
• Isotype: IgG1 kappa
• Host: Human