CloneID: AFA
Antigen Long Description: The original antibody was produced by immunizing mice with a synthetic peptide (GGGYDFGYDGDFYRA) whose sequence corresponds to that of the C-terminal telopeptide of the α2 chain (α2Ct).
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody is specific for human collagen I, showing high affinity to the GDF sequence (in A2_DGDFY, part of the α2(I)C-telopeptide), suggesting it is a critical epitope.
Application Notes (Clone): This antibody binds human collagen I and blocks the binding activity of its C-terminal telopeptide region (denoted as CTTR1) consisting of two α1(I)C-telopeptides (denoted as α1Ct) and one α2(I)C-telopeptide (denoted as α2Ct). The KD of this antibody and its Fab and scFv with native α2Ct present in procollagen I and with synthetic α2Ct were 663 pM, 268 nM, 21 pM, 57 nM, and 75nM for full-length AFA/procollagen I, Fab AFA antibody/procollagen I, full-length AFA antibody/synthetic α2Ct, Fab AFA antibody/synthetic α2Ct, and scFv/procollagen I, respectively (US20200048333A1). A chimeric IgG version of the original IgA version of this antibody was engineered (Fertala et al., 2013; PMID: 23586407). This antibody has been shown to be an effective therapeutic tool for blocking collagen fibril formation in various models (Fertala et al., 2017; PMID: 28972447) (Fertala et al., 2017; PMID: 23586407).
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