CloneID: 3G11
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with type IV collagenase.
Origin Pub PMID: 20303650
Buffer Composition: PBS only.
Chimeric Use Statement: This is a reformatted mouse IgG2a Fc Silent™ antibody, based on the original mouse IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P33434
Specificity Statement: This antibody is specific for type IV collagenase.
Application Notes (Clone): The immunoreactivity of the original format of this antibody (mouse IgG1, κ) was measured via ELISA and was shown to decrease by approximately 10%-20% after cold iodination with 131-iodide. The (131)I-labeled version of this antibody was demonstrated to be stable in vitro for almost 72 h. The blood clearance pattern of (125)I-labeled 3G11 was defined as a two-compartment model, with a 7.2 h T1/2α and a 345.2 h T1/2β, and clear scintigraphic images of human lung carcinoma PG were obtained using (125)I-labeled 3G11 at 72 h, which further improved at 120 h (Dai et al., 2003; PMID: 14693044). Lidamycin (LDM) was conjugated to the Fab version of this antibody, and the resulting immunoconjugate maintained most of its immunoreactivity to both type IV collagenase and mouse hepatoma 22 cells, as shown by ELISA, and exhibited more potent cytotoxicity to hepatoma 22 cells than free LDM by MTT assay. Furthermore, when administered intravenously, the Fab'-LDM conjugate was proven to be more effective against the growth of subcutaneously transplanted hepatoma 22 in mice than free LDM in two experiment settings, increasing mice survival time (Fengqiang et al., 2004; PMID: 15382678). The scFv version of this antibody was constructed and used in various configurations for IF and in vivo imaging and therapeutic applications (Zhong et al., 2010; PMID: 20303650) (Kong et al., 2015; PMID: 25824464).
English
Deutsch
