CloneID: 3H9
Antigen Long Description: The original antibody was generated by immunizing Balb/c mice with a mixture of six Cry1 toxins (Cry1Aa, Cry1Ab, Cry1Ac, Cry1B, Cry1C, and Cry1F) followed by antibody library construction and phage display-based selection.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This full-length reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q03746; P0A366; P0A370; P05068; D0EI56; Q93T75
Specificity Statement: The antibody binds to Cry1 toxins (Cry1Aa, Cry1Ab, Cry1Ac, Cry1B, Cry1C, Cry1F). The antibody does not cross react with Cry2A and vip3.
Application Notes (Clone): The specificity of the scFv antibody for the six Cry1 toxins was confirmed by ELISA. The scFv fragment was used to establish the double antibody sandwich enzyme-linked immunosorbent assay method (DAS-ELISA) for detecting six Cry1 toxins (Cry1Aa, Cry1Ab, Cry1Ac, Cry1B, Cry1C, and Cry1F). The lowest detectable limits (LOD) and the lowest quantitative limits (LOQ) were 3.14–11.07 and 8.22–39.44 ng mL−1 , respectively, with the correlation coefficient higher than 0.997. The average recoveries of Cry1 toxins from spiked rice leaf samples were ranged from 84 to 95%, with coefficient of variation (CV) less than 8.2%, showing good accuracy for the multi-residue determination of six Cry1 toxins in agricultural samples (Dong et al., 2018; PMID: 29484477).
English
Deutsch
