CloneID: NB25B-17
Antigen Long Description: The original antibody was generated by immunizing alpaca with human CTLA-4, followed by antibody library construction and phage display-based selection.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P16410
Specificity Statement: The antibody is specific for CTLA-4. CTLA-4 is a protein receptor that functions as an immune checkpoint and downregulates immune responses.
Application Notes (Clone): The binding affinity of humanized VHH-Fc chimeric antibody to human CTLA-4 expressed in CHO cells was shown by FACS experiments. The blocking activity of the humanized VHH-Fc chimeric antibody to the binding of CTLA-4 to CD80 or CD86 was shown using FACS. The antitumor activity of the humanized antibody was demonstrated in vivo in animal models. Specifically, a tumor animal model was established by inoculating mice with human breast cancer cells MDA-MB-231 (ATCC) to induce breast cancer. Mice were grouped and administered with the humanized version of the antibody, and tumor volume in tumor-bearing animals was measured periodically in size. Results showed that the humanized version of the antibody was able to significantly reduce tumor volume in tumor-bearing mice (CN113121689).
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