CloneID: 2.7D5
Antigen Long Description: The original antibody — a secondary immune response antibody — was generated by immunizing BALB/c mice with pigeon cytochrome C covalently coupled to ovalbumin using glutaraldehyde (CYT-OVA) and later challenging them with the same immunogen (pigeon CYT-OVA).
Buffer Composition: PBS only.
Uniprot Accession No.: P62897
Specificity Statement: This antibody is specific for pigeon cytochrome c (CYT) and cross-reacts with mouse CYT. Specifically, it recognizes the region around residue 62 of native mouse CYT.
Application Notes (Clone): The binding specificity of the original format of this antibody (mouse IgG2b) was evaluated by indirect ELISA, and its binding affinity was determined by competitive ELISA. It was found to bind Pigeon CYT ~5.6 times better than Mouse CYT (Liu et al., 2000; PMID: 11257306). This antibody was used in the detection of intracellular mouse cytochrome c (cyt c) in live DO11.10 T hybridoma cells by immunofluorescent (IF) labeling. Specifically, it was found to recognize the surface around residue 62 of cyt c and bound to cyt c in fixed and permeabilized cells. Furthermore, it was used in indirect IF staining of cyt c in post-apoptotic cells and immunoprecipitation (IP) of cyt c extracted from live, apoptotic, and necrotic DO11.10 cells (Jemmerson et al., 1999; PMID: 10090746). This antibody was used in immunogold labeling to determine the subcellular localization of cytochrome-c in rat pancreatic acinar cells (Soltys et al., 2001; PMID: 11319839).
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