CloneID: RCK107
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing a mouse with a cytoskeletal preparation of TR146 epithelial cells.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P02533
Specificity Statement: The antibody reacts exclusively with Cytokeratin 14 which is present in basal cell compartments of stratified and combined epithelia.
Application Notes (Clone): The antibody detected keratin-14 from cytoskeletal extracts were prepared from A431 and HaCaT cell cultures by western blot analysis. This antibody was used for immunohistochemistry studies of keratins on TR146, HaCaT and A431 cells. The antibody stained mostly basal (myoepithelial) cells (Wetzels et al., 1991; PMID: 1705754 and Smedts et al., 1992; PMID: 1379783). Further, the antibody stained mitochondria- rich cells (Spies et al., 1997; PMID: 9440221).
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