CloneID: RCK102
Antigen Long Description: The original antibody was generated by immunizing a mouse with cytokeratins from a Human lung cancer cell line (MR21).
Buffer Composition: PBS only.
Chimeric Use Statement: This is a chimeric rabbit IgG antibody, based on the original rabbit IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P05787; P13647
Specificity Statement: The original antibody binds to Cytokeratin 5 and 8.
Application Notes (Clone): The antibody recognized keratins 5 and 8, and as a result stained virtually all epithelial tissues. This antibody was used for immunohistochemistry on classic SCLC as well as the adenocarcinoma cell lines (Broers et al., 1986; PMID: 2433295). Further, endocervical columnar cells, cervical immature squamous metaplasia, cervical CIN I, CIN II and CIN III reacted with the antibody in immunohistochemistry (Smedts et al., 1990; PMID: 1690513). The antibody detected keratins 5 and 8 by western blot analysis on T 24, HeLa and KB cells (Ramaekers et al., 1987; PMID: 2436934).
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