CloneID: C9
Antigen Long Description: A chimeric library was created from clones that were able to bind DENV2 EDIII, this library was then panned against DENV2 EDIII to create this antibody.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P17763
Specificity Statement: This antibody is specific for the EDIII protein of the dengue virus. The dengue virus causes the disease known as dengue fever.
Application Notes (Clone): To determine the specificity and DENV cross-reactivity of the IgG1 version of this antibody an ELISA was performed on EDIII, this antibody had strong binding for EDIII from DENV2 and to a lesser extent with EDIII from DENV1 and DENV3. Furthermore, immunofluorescence was performed on uninfected BHK-21 cells and cells infected with DENV2 using the IgG1 version of this antibody, it showed clear fluorescence in the cytoplasm of infected cells. Finally, a plaque reduction neutralization test was performed using the IgG1 version of this antibody, this antibody showed a 25% reduction of plaque formation. This antibody is a chimeric antibody with mouse Vκ/human Cκ and mouse VH/human CH segments (Moreland et al, 2012; pmid:22489114).
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