CloneID: 2D12.5
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with KLH-21T-BAD-Y(III).
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody binds metal bound DOTA (Tetraxetan) and also shows binding affinity for Yttrium(III) labeled DOTA, (S) nitro benzyl-DOTA chelates and Janus-DOTA (a bivalent form of DOTA). DOTA is a chelating agent which can be labelled with radioactive yttrium ion and even conjugated with monoclonal antibodies for radioimmunotherapy.
Application Notes (Clone): The original mouse IgG1 antibody binds DOTA chelates of all the rare earths with approximately Kd ≈ 10(-8) M, making it useful for the capture of probe molecules with a variety of properties (PMID: 15546206). The original IgG1 version of this antibody was used in the pretargeting for radioimmunotherapy in BALB/c mice with KHJJ tumors (PMID: 7954426). In vivo clearance studies have demonstrated that 74-96% of 2D12.5 bound to metal chelates is present 24 hours after administration of the bound complex (US7528235). An engineered version of this antibody with a single cysteine residue at position 54, 55 or 56 of the heavy chain, a site proximal to the protein's binding site, enables formation of weakly electrophilic metal complexes of (S)-2-(4-acrylamidobenzyl)-DOTA (AABD) that may bind and form permanent linkages (PMID: 15546206; 15546207). The heavy chain G54C mutant had a small set of electrophilic ligands, differences in reactivity indicated that the substituents near the electrophilic atom can be important determinants of permanent binding (PMID: 15546207).
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