Anti-EGFR (7D12)

Anti-EGFR [7D12], Recombinant, IgG1-Fc Fusion, Human

CloneID: 7D12

Buffer Composition: PBS with 0.02% Proclin 300.

Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7

Uniprot Accession No.: P00533

Specificity Statement: This antibody is specific for the ligand binding site on domain III of the extracellular domain of human EGFR, overlapping with the epitope of cetuximab.

Application Notes (Clone): The binding of the original version of this antibody (VHH) to EGFR was characterized in an SPR (K_D 0.012 nM), ELISA, (EC₅₀ of 71.0 nM), and FACS (EC₅₀ of 0.79 nM). In its bi-specific construction with Ab04388 (Anti-CEACAM5 [NbCEA5]), it served a functional role (US20220242958).

• SKU: ABAAb04389-10.159
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab04389-10.159
• Package Unit: 100 μg
• Quantity Unit: STK
• Reactivity: Human
• Clonality: Recombinant
• Application: ELISA, Super-Resolution Microscopy
• Isotype: IgG1-Fc Fusion
• Host: Human