CloneID: ML66
Antigen Long Description: The original antibody was generated by immunizing female rats with membrane prep of MDA-MB468 breast cancer cell line.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P00533
Specificity Statement: The antibody is specific for EGFR.
Application Notes (Clone): The humanized version of the antibody exhibited strong specific binding to both human tumor cell lines and Vero cells by flow cytometry. This original and humanized version of the antibody were used for detection of EGFR expressed on MDA-MB468 cells by flow cytometry. The humanized version of the antibody induced NK cell mediated killing of target cells in a dose dependent manner. The chML66-antibody-maytansinoid conjugates significantly deleted the tumor growth in a tumor xenograft model using EGFR expressing KB cells implanted subcutaneously in SCID mice (US20160017053A1).
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