CloneID: M12B9
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was raised by immunizing a BALB/c mouse with live VACV and refolded L1 protein.
Buffer Composition: PBS only.
Chimeric Use Statement: This is a reformatted mouse IgG2a Fc Silent™ antibody, based on the original mouse IgG2a format, created for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P07612
Specificity Statement: This antibody is specific for the L1 protein of the vaccina virus. Regions 25-34 and 113-131 of L1 are part of the epitope.
Application Notes (Clone): A chimeric scFv-Fc version of this antibody was used for an ELISA on recombinant protein L1 of the variola virus strain India-1967, on lysate of MV and EV forms of the vaccinia virus strain LIVP (RU2790134C1), and on overlapping linear biotinylated peptides (Kaever et al., 2014; PMID:25031354). The ability of this antibody to protect mice in vivo from VACV challenge was tested on BALB/c mice. The mice were injected with the antibody a day before they were infected with VACV-WR. The antibody is able to prevent death in mice, but it is not able to prevent weight loss (Matho et al., 2015; PMID:26325270). This antibody was used for a FACS based neutralization assay. Here Vero E6 cells were incubated with a mixture of antibody and VACV-GFP and then tested in flow cytometry (Kaever et al., 2014; PMID:25031354). This antibody was used for in vivo treatment of mice infected with VACV. It was shown that injection of this antibody after being infected with the virus lead to a 100% survival rate (Zaitseva et al., 2017; PMID:28495463).
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