CloneID: 2E3
Antigen Long Description: A synthetic, fully human antibody library was used for panning against RSV prefusion and post fusion F proteins. The original antibody against RSV F protein was discovered following phage library panning and characterized.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P03420
Specificity Statement: The original antibody is specific for prefusion F protein. The antibody binds the protein through a conformational epitope spanning across three distinct sections of the RSV F protein: residues 419–434, 443–448, and 459–467. F-protein plays a pivotal role in the pathogenesis of RSV by mediating the fusion between the viral and the host cell membrane. F protein exists in two distinct conformations: the metastable prefusion conformation and the stable post fusion conformation.
Application Notes (Clone): The specificity of the Fab and IgG1 format of the antibody was confirmed by ELISA analysis (EC50 value for IgG1 at 9.6 ng/mL). Surface plasmon resonance based studies demonstrated that the antibody bound exclusively to the prefusion conformation of the RSV F protein with an affinity Kd = 13 nM for monovalent Fab. The bivalent Fab exhibited neutralizing activities against both RSV A (Long) and B (Washington) strains with IC50 at 53.4 ng/mL and 515 ng/mL, respectively. The human IgG1 format showed an improved neutralizing activity against the RSV A strain (IC50 at 11.4 ng/mL) and comparable against the RSV B (Washington) strain (IC50 at 520 ng/mL).
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