CloneID: mAb 338
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with a combination of some or all of the following: intranasal infection with hMPV at 10^6 TCID50 per animal of either NL1700, NL100, or NL199; intranasal infection with 10^6 PFU b/hPIV3/hMPV FNL100; intraperitoneal injection with adenovirus-vectored hMPV FNL100 or hMPV FNL199 at a dose of 9 × 10^7 TCID50; purified soluble hMPV F protein derived from NL100 and NL199 sequences injected intraperitoneally with either GERBU MM adjuvant.
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: Q6WB98
Specificity Statement: This antibody recognizes the hMPV F protein (a.a. 238-245). Human metapneumovirus (hMPV) is a member of the Paramyxoviridae family/Pneumovirinae subfamily and shares many common features with respiratory syncytial virus (RSV), another member of the same subfamily. hMPV causes respiratory tract illnesses that, similar to human RSV, occur predominantly during the winter months and have symptoms that range from mild to severe cough, bronchiolitis, and pneumonia.
Application Notes (Clone): ELISA was used to confirm the binding of this antibody to hMPV F protein. This antibody has been reported to have high neutralizing activity against all of the hMPV types A1, A2, B1, and B2 with an in vitro IC50 of < 2µg/ml. The antibody is effective as passive prophylaxis in protecting hamsters from HMPV challenge and also reduces incidence of MPV infection and disease in animal models (PMID: 16873237). Intraperitoneal injection of thia antibody into BALB/c mice significantly decreased lung viral titers after HMPV challenge when the antibody was administered prophylactically or therapeutically (PMID: PMID: 20619294).
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