CloneID: Af1 8
Antigen Long Description: This antibody was generated by panning a phage library prepared from 40 different samples of human peripheral blood lymphocytes against the F1 protein.
Buffer Composition: PBS only.
Chimeric Use Statement: This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P26948
Specificity Statement: This antibody is specific for the F1 capsule protein from Yersinia pestis. It shows some cross-reaction with other Yersinia species that have a F1 protein. F1 is a protein that is part of the capsule of Yersinia pestis. Yersinia pestis is the bacteria that causes the plague in humans.
Application Notes (Clone): To determine the ability of the scFv version of this antibody to bind the F1 protein, an ELISA was performed on F1 using the scFv version of this antibody, it showed strong binding. Furthermore, an ELISA was also performed on Yersinia pestis to assess the binding in vivo. Additionally, flow cytometry was performed on both Yersinia pestis and the F1 antigen, it showed a positive reaction to both (Lillo et al, 2011; pmid:22174746).
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