CloneID: clone4
Antigen Long Description: The recombinant fHbp protein of Neisseria meningitidis was used as the antigen for the immunization of BALB/c mice. Anti-fHbp VH/k chain ribosome display libraries was generated and the actual antibody was obtained by panning the scFv library against the recombinant fHbp protein by using a single round of the ribosome display method via a rabbit reticulocyte lysate system.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: E6MV22
Specificity Statement: This antibody binds a surface exposed epitope on N. meningitidis FHbp, a virulence factor critical for surviving of the bacteria in the human host. FHbp protein is a bacterial surface lipoprotein that binds host (human) complement factor H (fH, gene CFH). Neisseria meningitidis is the leading global cause of meningitis and sepsis.
Application Notes (Clone): This antibody exhibited high affinity and specificity in the enzyme-linked immunosorbent assay (ELISA) and the whole bacterial cell enzyme-linked immunosorbent assay (Bact-ELISA). The binding affinity of the scFv version of this antibody for fHbp was reported to be Kd= 7.03 nM (PMID: 29737965).
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