CloneID: KM33
Antigen Long Description: The original antibody was isolated from a human phage display library generated from the peripheral blood of an inhibitor patient and panning the library against factor VIII light chain.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P00451
Specificity Statement: This antibody binds the C1 domain of the human factor VIII, a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for factor IXa which converts factor X to the activated form Xa in the presence of calcium and phospholipids. Defects in the F8 gene, which encodes Factor VIII, result in the coagulation disorder hemophilia A.
Application Notes (Clone): The scFv version of this antibody was capable of inhibiting factor VIII activity with inhibition titre of 97 BU/mg. The scFv antibody binds human factor VIII light chain with a binding affinity of Kd= 0.1 nM. It was reported that scFv KM33 inhibits the procoagulant activity of factor VIII, although its epitope is located outside region R1803-K1818 (PMID: 11159524). It was reported that the presence of this antibody completely inhibited FVIII endocytosis by both monocyte and bone marrow derived mouse dendritic cells (PMID: 21962992). It was reported that this antibody inhibits FVIII activity and interactions with its physiological ligands, von Willebrand factor, phospholipids and catabolic receptors in vitro (PMID: 11159524; Limburg V et al., 2005).
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