Anti-Flavocytochrome B2 (B2B4)

Anti-Flavocytochrome B2 [B2B4], Recombinant, IgG1 lambda, Mouse

CloneID: B2B4

Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with fully purified Flb.

Buffer Composition: PBS only.

Uniprot Accession No.: P00175

Specificity Statement: The antibody recognized a conformational epitope on the Heme-binding domain of Flb2 (residues 1-99). Specifically, the epitope consists of Asn30, residues 63 to 65 (GluPro-Leu) and 67 to 70 (Ala-Pro-Asn-Val), Asp72, Tyr74, and the two heme propionate groups.

Application Notes (Clone): ELISA indicated that the antibody, either the IgG or the Fab fragment, was able to recognize the heme-binding domain of flavocytochrome b2. (PMID: 8674650) For the Fab fragment, Kd values of 80 nM and 30 nM were determined in ELISA competition experiments and fluorescence quenching titrations, respectively. (PMID: 9521665)

• SKU: ABAAb02944-1.1-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab02944-1.1-BT
• Package Unit: 1 mg
• Quantity Unit: STK
• Reactivity: Yeast
• Clonality: Recombinant
• Application: ELISA
• Isotype: IgG1 lambda
• Host: Mouse