CloneID: R5
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was isolated from peripheral blood mononuclear cell obtained from a patient diagnosed with Rift Valley fever.
Origin Pub PMID:
Buffer Composition: PBS only.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P03518
Specificity Statement: This antibody is specific for the Gc protein of Rift Valley fever virus (RVFV). The Gc protein is one of the two structural envelope glycoproteins of RVFV found on the virus's surface (the other being the glycoprotein N (Gn) protein). The Gc protein plays a crucial role in the entry of RVFV into host cells.
Application Notes (Clone): The original version of this antibody (human IgG1) was characterized using ELISA, SPR, and FACS assays to isolate it and assess its binding specificity and affinity. It bound its target with a Kd of 120 nM. In a FACS-based in vitro neutralization assay, this antibody exhibited a relatively low neutralization potency compared to its Gn-specific antibody counterparts with a median inhibitory concentration (IC50) of 1,370±530 ng/ml. However, it can still prevent RVFV infection at high concentrations. In an in vivo mouse model, mice treated with this antibody showed lower survival rates compared to those treated with Gn-specific antibodies. However, this antibody still contributed to clearing the virus, as evidenced by the survival of one mouse in the respective group, indicating that this antibody can neutralize RVFV infection at high doses also in vivo (Wang et al., 2019; PMID: 30936489).
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