CloneID: NA9D7
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was raised by immunizing a mouse with CVA16.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q9QF31
Specificity Statement: This antibody is specific for the inner surface of the canyon of CVA16.
Application Notes (Clone): The original antibody was used for a neutralization assay against CVA16 in human rhabdomyosarcoma (He et al., 2020; PMID:32027857). Further an ELISA was performed on 16 strains of CVA16 (He et al., 2020; PMID:32027857), and on CVA16 (Cu et al., 2021; PMID:32750255). The structure of this antibody was determined using cryo-EM (He et al., 2020; PMID:32027857). A bispecific version of this antibody was used for an immunofluorescence assay on EV71- and CA16-infected cells. Furthermore a bispecific version of this antibody was used for an ELISA on EV71 or CA16. Finally the bispecific version of this antibody was used for neutralization (Zhou et al., 2019; PMID:30419253).
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